Since phosphorus is contained in DNA but not amino acids, radioactive phosphorus-32 was used to label the DNA contained in the T2 phage. Radioactive sulfur-35 was used to label the protein sections of the T2 phage, because sulfur is contained in protein but not DNA.
Why did Hershey and Chase use the isotopes?
Hershey and Chase sought to determine if the replicating piece of phages that entered bacteria during infection, the genetic parts, were solely DNA. To perform their experiments, Hershey and Chase utilized a technique called radioactive isotope labeling.
Why did Hershey and Chase used radioactive sulfur and phosphorus in their experiment?
Hershey and Chase experimented with radioactively labeled phosphorus and sulphur to determine that DNA and not protein is the genetic material.
Why did Hershey and Chase used radioactive sulphur and radioactive phosphorus in their experiment write the conclusion they arrived at and how?
a)Hershey and Chase grew some viruses on a medium that contained radioactive phosphorus and some others on a medium that contained radioactive sulfur. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not.
Why did Hershey and Chase label the viral DNA with radioactive phosphorus?
DNA contains phosphorus but no sulfur. Protein contains sulfur but no phosphorus. (By growing viruses in separate cultures, they ensured that one sample had only radioactive DNA while the other had radioactive protein. This would allow them to conclude which material was injected by the virus into the bacterium.)
Why were radioactive sulfur and phosphorus used to label bacteriophage in Hershey and Chase’S experiments quizlet?
In their experiment Hershey and Chase labeled the bacteriophages with radioactive isotopes to see where the virus attacks. They use phosphorus 32 and sulfur 35 because proteins contain almost no phosphors and DNA contains almost no sulfur.
Why did Hershey and Chase use sulfur?
Hence Hershey – Chase used radioactive isotopes of sulphur S) and phosphorus (P) to keep separate track of viral protein and nucleic acid in culture medium. The expected result cannot be achieved, if radioactive carbon and nitrogen is used, since these molecules are present in both DNA and proteins.
Why did Hershey and Chase use radioactively Labelled phosphorous and sulphur only would they have got the same result if they use radiolabelled carbon and nitrogen?
Generally proteins contain sulphur but not phosphorous and nucleic acid (DNA) contains , phosphorous but not sulphur. Hence Hershey – Chase used radioactive isotopes of sulphur ( 35S) and phosphorus ( 32P) to keep separate track of viral protein and nucleic acid in culture medium.
What was the point of Hershey and Chase choosing to use radioactive labels for the DNA and cellular proteins in their work?
Why were phosphorus-32 and sulfur-35 perfect for what Hershey & Chase were investigating in their experiments? They were perfect because proteins contain almost no phosphorus and DNA contains no sulfur, so the radioactive isotopes would show up in the cells if the phosphorus-32 or sulfur-35 appeared.
How did Hershey and Chase label viral DNA and viral protein?
10. How did Hershey and Chase “label” viral DNA and viral protein so that they could be distinguished? Hershey and Chase used a radioactive isotope of sulfur to tag protein and a radioactive isotope of phosphorus to tag DNA.
